Device

Part:BBa_K173007:Design

Designed by: Lorenzo Pasotti, Letizia Diamante, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-15)

aTc inducible system (aTc->PoPS) with J23100 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used BioBrick Standard Assembly.

Source

BBa_J23100, 2009 DNA Distribution;

BBa_K173005, UNIPV-Pavia iGEM 2009 team.

References

Lutz R and Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res 1997 Mar 15; 25(6) 1203-10.